Pdf micropropagation of asparagus by in vitro shoot culture. Asparagus racemosus due to its slow growth faces lots of problem which can be solved through tissue culture. Its medicinal usage has been reported in the indian and british pharmacopoeias and in traditional systems of medicine such as ayurveda, unani, and siddha. Propagation of asparagus racemosus through tissue culture. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext.
Study of phytohormones effect on micropropagation and shoot induction in asparagus racemosus int j cur res rev, nov 2012 vol 04 22 page 48 in case of reproductive disease. Since the callus quality plays an important role in plantlet development, therefore, compact embryogenic callus was selected for further embryogenesis. Hence, there is an urgent necessity to safeguard this germplasm, and plant tissue culture has proved helpful in conserving threatened plant species. Regeneration of shoot buds and clonal multiplication of excised shoots through proliferation of nodal buds could be achieved by the use of iaa and bap in the medium. Rapid in vitro micro propagation of asparagus racemosus willd. Investigation of cytotoxicity and invitro antioxidant activity of asparagus racemosus root extract. Liliaceae is a pharmacologically acclaimed phytoestrogenic medicinal plant used for its immunomodulatory diwanay et al.
A significant increase in antibody titers was observed compared to animals that were untreated. Murashige and skoogs medium with 2, 4d and kinetin induced callus in the shoot segments of asparagus racemosus. Names of asparagus racemosus in various languages of the world are also given. Supplementing asparagus racemosus will aid digestion, since the plant has antiulcer effects. Asparagus racemosus an overview sciencedirect topics. Asparagus racemosus, traditionally known as shatavari means who possesses a hundred husbands or acceptable to many. Micropropagation of asparagus racemosus shatavari abstract. Part of the methods in molecular biology book series mimb, volume 547 asparagus racemosus willd. Conservation of medicinal plant asparagus racemosus. Micropropagation of asparagus racemosus shatavari asparagus racemosus shatavari is a plant with immense medicinal values. Mohommad shahid department of botany, davpg college, dehradun. The plants are being overharvested, so this species faces a real danger of becoming vulnerable in its natural habitat.
Micropropagation of asparagus by in vitro shoot culture. Conservation micropropagation introduction asparagus racemosus willd. Asparagus racemosus a medicinally important plant can be propagated through tissue culture technique which save lots of time and money. Micropropagation insures a good regular supply of medicinal plants, using minimum space and time prakash and staden, 2007. The plant is also known as shatavari which means she who has hundred husbands which indicates to the rejuvenative effect. Induction of somatic embryos in cultures of asparagus. Asparagus racemosus roots were successively extracted with the series of solvents, that is, hexane, ethanol and water, and also a saponin. In spite of being a rejuvenating herb it is beneficial in female infertility, as it increases libido. In vitro propagation of wild asparagus adscendens roxb. Asparagus racemosus have numerous medicinal usages which have been reported in the indian and british pharmacopoeias and in indigenous systems of medicine.
R2 1department of biotechnology, school of life sciences, vels. Asparagus racemosus leaf extract inhibits growth of uok. It will also aid the immune system when antibodies are fighting off a threat. In indian system of medicine asparagus racemosus is an important medicinal plant and its root paste or root juice has been used in various ailments and as health tonic 1,2. Asparagus racemosus herb uses, benefits, cures, side. It is reported to be recalcitrant to tissue culture practices. Shatavarin production from in vitro cultures of asparagus.
Learn more about asparagus racemosus uses, effectiveness, possible side effects, interactions, dosage, user ratings and products that contain asparagus racemosus. A induction of calli on shoot tips and nodal bud explants on ms medium supplemented with 4 mg l1 bap and 0. Pdf micropropagation and phytochemical analysis of asparagus. The multiple uses of this species have increased its commercial demand, resulting in overexploitation. Shatavari asparagus racemosus is a powerful therapeutic agent, which is of great importance in ayurveda. Micropropagation of asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis. Little work has been undertaken on in vitro culture in asparagus racemosus as compared to that of a. List of various diseases cured by asparagus racemosus. Asparagus officinalis is most extensively studied species within the genus asparagus, which is well known as garden asparagus. It is good source of folic acid, potassium, dietary fiber. Somatic embryogenesis is one of the most popular in vitro regeneration methods for mass micropropagation. B adventitious shoot formation from surface of calli on ms with 2 mg l1 and 0. Direct in vitro propagation of asparagus adscendens.
Studies using seeds as means of propagation indicated. The multiple uses of this species have increased its commercial demand. Micropropagation of asparagus densiflorus via axillary. Pdf micropropagation and phytochemical analysis of. Shatavari, also known as satavari, satavar, or asparagus racemosus a. In vitro propagation of a high value medicinal plant.
Teratogenicity of asparagus racemosus roots 571 methanol 1 l by cold percolation method for 5 days. Rapid in vitro protocol for high multiple shoot induction. Asparagaceae, commonly called shatavari, is a woody climber that grows throughout tropical and subtropical parts of india up to an altitude of 1,500 m. The therapeutic applications of this plant have been reported in indian and british pharmacopoeias and in traditional system of medicine, such as ayurveda, unani and siddha. This species is dioecious with unisexual flowers, which means that generative propagation gives roughly equal number of male and female plants. Plant tissue culture techniques have become a powerful tool for studying and solving basic and applied problems in plant biotechnology.
It has antioxidant activity, antiabortifacient activity shatvarin 1, antioxytoxic shatavarin4, spasmodic to uterus, hypoglycemic. There is always problem of heterogeneity in obtaining uniform seed stock. Pdf asparagus officinalis is most extensively studied species within the genus asparagus, which is well known as garden asparagus. Uses, benefits, cures, side effects, nutrients in asparagus racemosus. The majority of plants used by the pharmaceutical industry come from wild sources, endangering the natural population of the species. Micropropagation of asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis anna pindel a. Asparagus racemosus willd is multiplied through vegetative propagation in nature.
Asparagus racemosus is a commercially important medicinal plant, traditionally used for combating gynecological problems in india. The amounts of saponin equivalent to shatavarin iv in the extracts were determined using elisa. In vitro clonal propagation of asparagus racemosus, a high. Rapid in vitro multiplication of asparagus sciencedirect.
Senpropagation of asparagus racemosus through tissue culture. Asparagus racemosus is a well known ayurvedic rasayana which prevent ageing, increase longevity, impart immunity, improve mental function, vigor and add. Asparagus racemosus satavar, shatavari, or shatamull, shatawari is a species of asparagus common throughout india and the himalayas. Asparagus leaf extract inhibits growth of renal cell cancer cells asian pac j cancer prev, 15 5, 19371941 introduction asparagus racemosus willd. Regeneration of asparagus racemosus by shoot apex and. This plant is known to produce steroidal saponins called shatavarins. Kar dk and sen s 1985 propagation of asparagus racemosus through tissue culture. How asparagus racemosus is effective for various diseases is listed in repertory format. Shatavari asparagus racemosus is a wondrous plant that holds a special place in the heart of the ayurvedic pharmacopoeia. The roots are said to be tonic and diuretic and galactgogue, the drug has ulcer healing effect probably via strenthening the mucosal resistance or cytoprotection.
Asparagus racemosus is an indigenous medicinal plant of the family liliaceae 1956. Asparagus officinalis cultivar franklim was successfully propagated in vitro on a. Shatavari, micropropagation, tissue culture, in vitro culture. In the present study, somatic embryogenesis via zygotic embryos was studied in asparagus racemosus willd. The extracts were filtered, vacuum dried and stored in a refrigerator until further use. It contains various type of secondary metabolite active natural products or ingredients such as steroids, alkaloids, flavonoids, furan derivatives and essential oils. Asparagus racemosus supplement health benefits, dosage.
Asparagus racemosus and crataeva magna remain species with tremendous potential and although considerable work has been done to exploit the biological activity and medicinal applications of these plants, countless possibilities for investigation still remain in relatively newer areas of its function. Thanks to its cooling, soothing, nourishing, and rejuvenating work throughout the body, shatavari effectively balances both pitta and vata. Asparagus racemosus is an herb used in ayurveda medicine. Asparagus racemosus is one of the important medicinal plants found in india, china and other parts of the world. It grows 12 m 3 ft 3 in6 ft 7 in tall and prefers to take root in gravelly, rocky soils high up in piedmont plains, at 1,3001,400 m 4,3004,600 ft elevation. Pdf asparagus racemosus commonly known as shatavari in marathi and hindi. Effect of various concentrations of 2ip and coconut milk supplemented to ms medium on in vitro elongation of regenerated shoots of asparagus racemosus. In vitro plantlet regeneration in asparagus racemosus.
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